Upload larger files with python , Django and IIS server

I have file upload code in Django + python and I have my site register with IIS server. when running python code from command prompt on localhost:5000 and upload larger files (275 MB) is working perfectly.

but when runs from websitedomain which is set with IIS version 10 , error 500 page is coming.

I have set system.webServer/security/requestFiltering -> maxAllowedContentLength to 4294967295 -> maxQueryString 2048 and maxUrl 4096. I have also set to system.web/httpRuntime -> maxRequestLength to 2147483647 .

I am expecting to upload larger files. any help ? Thank you in Advance PB

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